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Differential effects of resveratrol on androgen-responsive LNCaP human prostate cancer cells in vitro and in vivo. F-1

                                             

Effects of resveratrol on LNCaP cell growth in vitro

                                

Effects of resveratrol on LNCaP cell growth in culture.

(A) Concentration-dependent effects of resveratrol on LNCaP cells cultured in 10% FBS. LNCaP cells were plated in 24-well plates; 24 h after plating, cells were treated with 0, 1, 5 or 25 μM resveratrol for 96 h and cell growth determined as described in Materials and Methods. Results are expressed as mean ± SD (n = 4). Bars with *are significantly different from vehicle control (0) at P < 0.05.

(B) Effects of resveratrol on R1881-induced cell growth. LNCaP cells were plated in 24-well plates; 24 h after plating, the medium was switched to Media B, which contains 10% CDS, for an additional 24 h. Cells were then treated with and without R1881 (1 nM) in the presence of 0, 1, 5 or 25 μM resveratrol for 96 h and cell growth determined as described in Materials and Methods. Results are expressed as percent inhibition (mean ± SD, n = 4). Bars with different letters are significantly different from each other at P < 0.05.

(C) Effects of resveratrol on 17β-estradiol-induced growth. LNCaP cells were seeded in 24-well plates; 24 h after plating, medium was switched to Media B, which contains 10% CDS, for an additional 24 h. Cells were then treated with and without 17β-estradiol (1 nM) in the presence of 0, 1, 5 and 25 μM resveratrol for 96 h, and cell growth was determined as described in Materials and Methods. Results are expressed as percent inhibition (mean ± SD, n = 4). Bars with different letters are significantly different from each other at P < 0.05.

Article Produced By

Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agriculture Research Service, United States Department of Agriculture, 10300 Baltimore Avenue, Building 307C, Room 132, Beltsville, MD. Laboratory of Cellular Regulation and Carcinogenesis, National Cancer Institutes, National Institutes of Health, Department of Nutrition and Food Science, University of Maryland, College Park, Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program College of Pharmacy, Washington State University, Pullman, National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan. Nutritional Sciences Research Group, Division of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Biometrical Consulting Service, Beltsville Area, Agriculture Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA. Division of Nutritional Sciences, University of Texas at Austin, Austin, TX 78712, USA. Department of Carcinogenesis, MD Anderson Cancer Center, Smithville, TX 78957, USA
 

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