(A) Concentration-dependent effects of resveratrol on LNCaP cells cultured in 10% FBS. LNCaP cells were plated in 24-well plates; 24 h after plating, cells were treated with 0, 1, 5 or 25 μM resveratrol for 96 h and cell growth determined as described in Materials and Methods. Results are expressed as mean ± SD (n = 4). Bars with *are significantly different from vehicle control (0) at P < 0.05.
(B) Effects of resveratrol on R1881-induced cell growth. LNCaP cells were plated in 24-well plates; 24 h after plating, the medium was switched to Media B, which contains 10% CDS, for an additional 24 h. Cells were then treated with and without R1881 (1 nM) in the presence of 0, 1, 5 or 25 μM resveratrol for 96 h and cell growth determined as described in Materials and Methods. Results are expressed as percent inhibition (mean ± SD, n = 4). Bars with different letters are significantly different from each other at P < 0.05.
(C) Effects of resveratrol on 17β-estradiol-induced growth. LNCaP cells were seeded in 24-well plates; 24 h after plating, medium was switched to Media B, which contains 10% CDS, for an additional 24 h. Cells were then treated with and without 17β-estradiol (1 nM) in the presence of 0, 1, 5 and 25 μM resveratrol for 96 h, and cell growth was determined as described in Materials and Methods. Results are expressed as percent inhibition (mean ± SD, n = 4). Bars with different letters are significantly different from each other at P < 0.05.
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