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Differential effects of resveratrol on androgen-responsive LNCaP human prostate cancer cells in vitro and in vivo. F-5

    

IHC analysis of markers for the steroid hormone-responsive pathway

    

IHC analysis of markers for the steroid hormone-responsive pathway,

proliferation, apoptosis and angiogenesis. IHC analyses for the steroid hormone-responsive gene PSA, the proliferation marker PCNA, apoptosis and the angiogenesis marker PECAM-1 were performed on paraffin-embedded tumor samples as described in Materials and Methods.

(A) PSA. PSA expression index = average PSA-positive cells/mm2

(B) PCNA. Proliferation index = average PCNA-positive cells/mm2

(C) ApopTag. Apoptosis index = average apoptotic cells per field

(D) PECAM-1. Angiogenesis index = average vessel counts per field *indicates significantly different from control at P < 0.05.

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Article Produced By

Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agriculture Research Service, United States Department of Agriculture, 10300 Baltimore Avenue, Building 307C, Room 132, Beltsville, MD. Laboratory of Cellular Regulation and Carcinogenesis, National Cancer Institutes, National Institutes of Health, Department of Nutrition and Food Science, University of Maryland, College Park, Department of Pharmaceutical Sciences and Pharmacology and Toxicology Graduate Program College of Pharmacy, Washington State University, Pullman, National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan. Nutritional Sciences Research Group, Division of Cancer Prevention, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. Biometrical Consulting Service, Beltsville Area, Agriculture Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA. Division of Nutritional Sciences, University of Texas at Austin, Austin, TX 78712, USA. Department of Carcinogenesis, MD Anderson Cancer Center, Smithville, TX 78957, USA
 

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