x
To determine the prostate cancer protective effects of resveratrol in vivo, a nude mouse xenograft model was used. Five-week-old male athymic nude mice (BALB/cAnNCr-nu/nu, 20–22 g; Charles River, Frederick, MD) were individually housed in filter-top cages at the Beltsville Human Nutrition Research Center animal facility. Animals were randomly assigned to the following diet groups:
(i) control (AIN-93M) diet,
(ii) AIN-93M supplemented with 50 mg resveratrol/kg diet (RES50) or
(iii) AIN-93M supplemented with 100 mg resveratrol/kg diet (RES100).
Pellet diets were prepared by Research Diets (New Brunswick, NJ). The concentrations of resveratrol were selected based on published effective doses for cancer prevention and those used in prior animal studies. Twenty-two mice were used per treatment group. After 2 weeks of adaptation on the diets, human LNCaP prostate tumors were established in the animals by a subcutaneous injection of LNCaP cells (2 × 106 cells) resuspended in 50 μl of phosphate-buffered saline (PBS) (pH 7.4) plus 50 μl of Matrigel (BD Biosciences, Bedford, MA).
The animals were palpated for tumors, and measurement of tumor volume began on the third week after injection. The cancer preventive efficacy of resveratrol was assessed by twice-weekly measurements of tumor volume, which was calculated using the equation: tumor volume (cm3) = 0.523 × [length (cm) × width2 (cm2)]. Mice were fed the diets described above until 7 weeks after the injection of cells, at which time the animals were euthanized.
Tumors were removed from the animals and a sample of tumor tissue was fixed in 10% neutral buffered formalin, embedded in paraffin and cut into 5 μm sections for in situ immunohistochemical (IHC) analysis. Remaining tumor samples were flash frozen under liquid nitrogen and stored at −80°C for resveratrol analysis as described below. Plasma samples were collected and stored at −80°C using the BD Vacutainer® PPT™ Plasma Preparation Tube Procedure as provided by the manufacturer (BD Biosciences, San Jose, CA).
Article Produced By
Posted by
Chuck Reynolds
The reasoning for this article is to supply you information about this wonderful product and also for me to find at least two or more people for this simply Amazing Opportunity.. You just need to follow the 4 simple steps.. (Join,Train,sigup 2 or more people and then you duplicate. It's that simple..). This business can make you money in the thousands or just be a customer for a very satisfying experience.
View this video as it helps you to understand a very simple Business Plan. Check it out, then
View these videos (4 of them, very short (total less then 5 min)) that explains how simple
this business works and if so inclined, click on my name above, call me or just sign up
either as a Customer or Distributor.
