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GFP_LC3 HeLa was generated as previously described. The above-mentioned cells and HEK293 cells were maintained with DMEM (Dulbecco’s modified Eagle’s medium) complemented with 10% fetal bovine serum (FBS, Lonza). To measure mTOR activity upon resveratrol treatment, cells were pretreated with 50 μM resveratrol (Sigma-Aldrich) for 30 min before stimulation with insulin or amino acids. Amino acid or serum fasting was conducted by maintaining cells with HBSS (Hank’s balanced salt solution; Invitrogen) supplemented with 10% dialyzed FBS (Invitrogen) or DMEM only, respectively. Before the resveratrol treatment, 10 μM H-89 (Biomol) was add for 1 h. For autophagy formation analysis using Western blotting, 10 μM of resveratrol was treated into cells in the presence or absence of 10 μM of Bafilomycin A1 (Enzo) for 2 h and cells were subjected to SDS-PAGE and Western blotting.
Article Produced By
Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea
School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan 689-798, Republic of Korea
Department of Bioscience and Biotechnology, Sejong University, 98 Kunja-Dong, Kwangjin-Ku, Seoul 143-747, Republic of Korea
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4763238/
(All these Articles can be read from this URL:
https://markethive.com/group/ama/blog/the-role-of-resveratrol-in-achieving-optimal-health-p5 )
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